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Analyte molecules partition involving a liquid stationary section plus the eluent. Equally as in hydrophilic interaction chromatography (HILIC; a sub-procedure within just HPLC), this process separates analytes based on distinctions inside their polarity. HILIC most frequently employs a bonded polar stationary stage plus a mobile stage manufactured
While in the separation, the molecules managing through the column can be regarded as getting inside a steady equilibrium amongst the cellular section and also the stationary section. This equilibrium could be ruled by an equilibrium consistent K, outlined as ref eight , where Cmo is the molar focus of your molecules in the mobile stage, and Cst co